Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria.
Identifieur interne : 000535 ( Main/Exploration ); précédent : 000534; suivant : 000536Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria.
Auteurs : Katrin Kaufmann [Allemagne] ; Luke Simmons ; Jennifer Herrmann ; Gertrud Schw R ; Nora Luniak ; Rolf MüllerSource :
- Biotechnology letters [ 1573-6776 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Anti-Inflammatory Agents (metabolism), Anti-Inflammatory Agents (pharmacology), Biological Assay (methods), Biological Products (metabolism), Biological Products (pharmacology), CHO Cells, Cell Line, Cricetinae, Cricetulus, Drug Evaluation, Preclinical (methods), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Hydroxysteroids (metabolism), Hydroxysteroids (pharmacology), Mice, NF-kappa B (genetics), NF-kappa B (metabolism), Reactive Oxygen Species (metabolism), Receptors, Glucocorticoid (genetics), Receptors, Glucocorticoid (metabolism), Transfection.
- MESH :
- chemical , genetics : Green Fluorescent Proteins, NF-kappa B, Receptors, Glucocorticoid.
- chemical , metabolism : Anti-Inflammatory Agents, Biological Products, Green Fluorescent Proteins, Hydroxysteroids, NF-kappa B, Reactive Oxygen Species, Receptors, Glucocorticoid.
- chemical , pharmacology : Anti-Inflammatory Agents, Biological Products, Hydroxysteroids.
- methods : Biological Assay, Drug Evaluation, Preclinical.
- Animals, CHO Cells, Cell Line, Cricetinae, Cricetulus, Mice, Transfection.
Abstract
Using an in vitro cell-based assay in a flow-design, we have applied activity-guided screening to search for new bioactive compounds isolated from microorganisms. A first assay employs the stable expression of nuclear factor kappa B (NF-κB) while a second assay utilizes the glucocorticoid receptor (GR) coupled to green fluorescent protein. A specialized assay was implemented for both the translocation of NF-κB and to inhibit the translocation of cytokine-mediated NF-κB. In addition, we developed in a wide palette of cell lines used for a highly specialized GR-translocation assay to detect anti-inflammatory effects. This approach demonstrates the straight-forward combination of cell-based assays arranged with an automated fluorescence microscope. This allows for the direct sorting of extracts which are acting in a pharmaceutically interesting way. Initial results using this technique have led to the detection of new anti-inflammatory steroids from bacterial crude extracts.
DOI: 10.1007/s10529-012-1042-0
PubMed: 22983717
Affiliations:
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Le document en format XML
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Simmons</name></author><author><name sortKey="Herrmann, Jennifer" sort="Herrmann, Jennifer" uniqKey="Herrmann J" first="Jennifer" last="Herrmann">Jennifer Herrmann</name></author><author><name sortKey="Schw R, Gertrud" sort="Schw R, Gertrud" uniqKey="Schw R G" first="Gertrud" last="Schw R">Gertrud Schw R</name></author><author><name sortKey="Luniak, Nora" sort="Luniak, Nora" uniqKey="Luniak N" first="Nora" last="Luniak">Nora Luniak</name></author><author><name sortKey="Muller, Rolf" sort="Muller, Rolf" uniqKey="Muller R" first="Rolf" last="Müller">Rolf Müller</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:22983717</idno><idno type="pmid">22983717</idno><idno type="doi">10.1007/s10529-012-1042-0</idno><idno type="wicri:Area/PubMed/Corpus">000447</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000447</idno><idno 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wicri:level="3"><nlm:affiliation>Department of Pharmaceutical Biotechnology, Saarland University, Saarbrücken, Germany. kaufm@uni-trier.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pharmaceutical Biotechnology, Saarland University, Saarbrücken</wicri:regionArea><placeName><region type="land" nuts="2">Sarre (Land)</region><settlement type="city">Sarrebruck</settlement></placeName></affiliation></author><author><name sortKey="Simmons, Luke" sort="Simmons, Luke" uniqKey="Simmons L" first="Luke" last="Simmons">Luke Simmons</name></author><author><name sortKey="Herrmann, Jennifer" sort="Herrmann, Jennifer" uniqKey="Herrmann J" first="Jennifer" last="Herrmann">Jennifer Herrmann</name></author><author><name sortKey="Schw R, Gertrud" sort="Schw R, Gertrud" uniqKey="Schw R G" first="Gertrud" last="Schw R">Gertrud Schw R</name></author><author><name sortKey="Luniak, Nora" sort="Luniak, Nora" uniqKey="Luniak N" first="Nora" last="Luniak">Nora Luniak</name></author><author><name sortKey="Muller, Rolf" sort="Muller, Rolf" uniqKey="Muller R" first="Rolf" last="Müller">Rolf Müller</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Anti-Inflammatory Agents (metabolism)</term><term>Anti-Inflammatory Agents (pharmacology)</term><term>Biological Assay (methods)</term><term>Biological Products (metabolism)</term><term>Biological Products (pharmacology)</term><term>CHO Cells</term><term>Cell Line</term><term>Cricetinae</term><term>Cricetulus</term><term>Drug Evaluation, Preclinical (methods)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Hydroxysteroids (metabolism)</term><term>Hydroxysteroids (pharmacology)</term><term>Mice</term><term>NF-kappa B (genetics)</term><term>NF-kappa B (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Receptors, Glucocorticoid (genetics)</term><term>Receptors, Glucocorticoid (metabolism)</term><term>Transfection</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>NF-kappa B</term><term>Receptors, Glucocorticoid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anti-Inflammatory Agents</term><term>Biological Products</term><term>Green Fluorescent Proteins</term><term>Hydroxysteroids</term><term>NF-kappa B</term><term>Reactive Oxygen Species</term><term>Receptors, Glucocorticoid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Inflammatory Agents</term><term>Biological Products</term><term>Hydroxysteroids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biological Assay</term><term>Drug Evaluation, Preclinical</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cell Line</term><term>Cricetinae</term><term>Cricetulus</term><term>Mice</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using an in vitro cell-based assay in a flow-design, we have applied activity-guided screening to search for new bioactive compounds isolated from microorganisms. A first assay employs the stable expression of nuclear factor kappa B (NF-κB) while a second assay utilizes the glucocorticoid receptor (GR) coupled to green fluorescent protein. A specialized assay was implemented for both the translocation of NF-κB and to inhibit the translocation of cytokine-mediated NF-κB. In addition, we developed in a wide palette of cell lines used for a highly specialized GR-translocation assay to detect anti-inflammatory effects. This approach demonstrates the straight-forward combination of cell-based assays arranged with an automated fluorescence microscope. This allows for the direct sorting of extracts which are acting in a pharmaceutically interesting way. Initial results using this technique have led to the detection of new anti-inflammatory steroids from bacterial crude extracts.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Sarre (Land)</li></region><settlement><li>Sarrebruck</li></settlement></list><tree><noCountry><name sortKey="Herrmann, Jennifer" sort="Herrmann, Jennifer" uniqKey="Herrmann J" first="Jennifer" last="Herrmann">Jennifer Herrmann</name><name sortKey="Luniak, Nora" sort="Luniak, Nora" uniqKey="Luniak N" first="Nora" last="Luniak">Nora Luniak</name><name sortKey="Muller, Rolf" sort="Muller, Rolf" uniqKey="Muller R" first="Rolf" last="Müller">Rolf Müller</name><name sortKey="Schw R, Gertrud" sort="Schw R, Gertrud" uniqKey="Schw R G" first="Gertrud" last="Schw R">Gertrud Schw R</name><name sortKey="Simmons, Luke" sort="Simmons, Luke" uniqKey="Simmons L" first="Luke" last="Simmons">Luke Simmons</name></noCountry><country name="Allemagne"><region name="Sarre (Land)"><name sortKey="Kaufmann, Katrin" sort="Kaufmann, Katrin" uniqKey="Kaufmann K" first="Katrin" last="Kaufmann">Katrin Kaufmann</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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